免疫相关基因数量到底是多少个

种数据挖掘文章本质上都是要把目标基因集缩小，比如表达量矩阵通常是2万多个蛋白编码基因，不管是表达芯片还是RNA-seq测序的，采用何种程度的差异分析，最后都还有成百上千个目标基因。如果是临床队列，通常是会跟生存分析进行交集，或者多个数据集差异结果的交集，比如：多个数据集整合神器-RobustRankAggreg包，这样的基因集就是100个以内的数量了，但是仍然有缩小的空间，比如lasso等统计学算法，最后搞成10个左右的基因组成signature即可顺利发表。

其实还有另外一个策略，有点类似于人工选择啦，通常是可以往热点靠，比如肿瘤免疫，相当于你不需要全部的两万多个基因的表达量矩阵进行后续分析， 仅仅是拿着几千个免疫相关基因的表达矩阵即可。

但是关于这一点，就有很多粉丝问我，为什么看了很多文献，大家的 免疫相关基因集的数量都不一样，希望我给出一个可靠的数据源！

随便列举几个免疫基因集数据挖掘文献：

2020-Development and Validation of a Novel Immune-Related Prognostic Model in Hepatocellular Carcinoma

A total of 329 differentially expressed immune-related genes were detected.
64 immune-related genes were identified to be markedly related to overall survival in HCC patients using univariate Cox regression analysis.

2020-A novel immune-related genes prognosis biomarker for melanoma: associated with tumor microenvironment

63 immune-related genes of the total 1039 unique IRGs retrieved were associated with overall survival of melanoma.

2020-Immune-related gene signature for predicting the prognosis of head and neck squamous cell carcinoma

Among 1073 immune genes measured on all platforms, 915 IRGs were selected after filtering median absolute deviation (MAD) > 0.5.
By 1000 times resampling, 81 IRGs were robustly associated with individual patients’ OS.

2020-Novel Immune-Related Gene Signature for Risk Stratification and Prognosis of Survival in Lower-Grade Glioma

277 immune-related DEGs were identified
6 immune genes ( CANX , HSPA1B , KLRC2 , PSMC6 , RFXAP , and TAP1 ) were identified as risk signature

你可以迅速解读一波，因为都大同小异，仅仅是癌症不一样，图表没啥子区别。差异分析，火山图，热图等等标准流程，基本上读一下我在生信技能树的表达芯片的公共数据库挖掘系列推文就明白了；

免疫基因集数据库

其实可以看到， 大多就是来源于 ImmPort（The Immunology Database and Analysis Portal）数据库的：

A predictive immune-related signature was constructed by concentrating on immune-related genes (IRGs) obtained from the Immunology Database and Analysis Portal (ImmPort) (https://immport.niaid.nih.gov).
A comprehensive list of IRGs was downloaded from the Immunology Database and Analysis Portal (ImmPort) database2. The list comprised a total of 2,498 IRGs, covering 17 immune categories(Bhattacharya et al., 2014).

如果你是初出茅庐，就选择它好了，反正数据库都提供了列表：https://www.immport.org/shared/genelists